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conventional wide field fluorescence microscopy imaging  (Nikon)
99
Nikon conventional wide field fluorescence microscopy imaging
Visualization and model of TAP activity. ( A ) <t>Fluorescence</t> <t>microscopy</t> images of time-lapse experiments in a microfluidic chamber, showing TAPs’ transfer between a donor carrying the RP4 helper and the indicated TAP, and the E2 recipient strains. Real-time monitoring allows the visualization of the effect of TAP acquisition in the absence and presence of exposure to Ctx treatment. Arrows indicate Ctx S TAP donors producing both red and green fluorescence (red D), and Ctx R recipients producing no fluorescence (white R). TAP acquisition is reflected by the production of green fluorescence in transconjugant cells (green T). Time in minutes, with 0 min corresponding to the time of Ctx injection. Scale bar 1 μm. ( B ) Model for TAP activity against strains carrying bla CTX-M-15 . The donor strain transfers a TAP carrying cas9 or dcas9 genes through the conjugation machinery provided by the helper plasmid. The recipient cell having acquired the TAP produces the components of the CRISPR system. The Cas9 or the dCas9 protein is recruited to the targeted bla CTX-M-15 gene by the complementary gRNA. The Cas9 induces a DSB, while the dCas9 inhibits the expression of the bla CTX-M-15 gene. The subsequent effect strictly depends on the chromosomic or plasmidic location of the bla CTX-M-15 gene. TAP-Cas9 targeting the chromosome leads to cell death. TAP-Cas9 targeting a plasmid results in plasmid loss, which can lead to cell death if some TA systems are activated. TAP-dCas9 targeting the chromosome or a plasmid does not affect viability but results in resensitization of the recipient to the drug.
Conventional Wide Field Fluorescence Microscopy Imaging, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vs120 upright wide field fluorescence microscope  (Olympus)
99
Olympus vs120 upright wide field fluorescence microscope
Visualization and model of TAP activity. ( A ) <t>Fluorescence</t> <t>microscopy</t> images of time-lapse experiments in a microfluidic chamber, showing TAPs’ transfer between a donor carrying the RP4 helper and the indicated TAP, and the E2 recipient strains. Real-time monitoring allows the visualization of the effect of TAP acquisition in the absence and presence of exposure to Ctx treatment. Arrows indicate Ctx S TAP donors producing both red and green fluorescence (red D), and Ctx R recipients producing no fluorescence (white R). TAP acquisition is reflected by the production of green fluorescence in transconjugant cells (green T). Time in minutes, with 0 min corresponding to the time of Ctx injection. Scale bar 1 μm. ( B ) Model for TAP activity against strains carrying bla CTX-M-15 . The donor strain transfers a TAP carrying cas9 or dcas9 genes through the conjugation machinery provided by the helper plasmid. The recipient cell having acquired the TAP produces the components of the CRISPR system. The Cas9 or the dCas9 protein is recruited to the targeted bla CTX-M-15 gene by the complementary gRNA. The Cas9 induces a DSB, while the dCas9 inhibits the expression of the bla CTX-M-15 gene. The subsequent effect strictly depends on the chromosomic or plasmidic location of the bla CTX-M-15 gene. TAP-Cas9 targeting the chromosome leads to cell death. TAP-Cas9 targeting a plasmid results in plasmid loss, which can lead to cell death if some TA systems are activated. TAP-dCas9 targeting the chromosome or a plasmid does not affect viability but results in resensitization of the recipient to the drug.
Vs120 Upright Wide Field Fluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wide field microscopy  (Photon Etc)
86
Photon Etc wide field microscopy
Visualization and model of TAP activity. ( A ) <t>Fluorescence</t> <t>microscopy</t> images of time-lapse experiments in a microfluidic chamber, showing TAPs’ transfer between a donor carrying the RP4 helper and the indicated TAP, and the E2 recipient strains. Real-time monitoring allows the visualization of the effect of TAP acquisition in the absence and presence of exposure to Ctx treatment. Arrows indicate Ctx S TAP donors producing both red and green fluorescence (red D), and Ctx R recipients producing no fluorescence (white R). TAP acquisition is reflected by the production of green fluorescence in transconjugant cells (green T). Time in minutes, with 0 min corresponding to the time of Ctx injection. Scale bar 1 μm. ( B ) Model for TAP activity against strains carrying bla CTX-M-15 . The donor strain transfers a TAP carrying cas9 or dcas9 genes through the conjugation machinery provided by the helper plasmid. The recipient cell having acquired the TAP produces the components of the CRISPR system. The Cas9 or the dCas9 protein is recruited to the targeted bla CTX-M-15 gene by the complementary gRNA. The Cas9 induces a DSB, while the dCas9 inhibits the expression of the bla CTX-M-15 gene. The subsequent effect strictly depends on the chromosomic or plasmidic location of the bla CTX-M-15 gene. TAP-Cas9 targeting the chromosome leads to cell death. TAP-Cas9 targeting a plasmid results in plasmid loss, which can lead to cell death if some TA systems are activated. TAP-dCas9 targeting the chromosome or a plasmid does not affect viability but results in resensitization of the recipient to the drug.
Wide Field Microscopy, supplied by Photon Etc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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wide field microscopy  (Olympus)
99
Olympus wide field microscopy
Visualization and model of TAP activity. ( A ) <t>Fluorescence</t> <t>microscopy</t> images of time-lapse experiments in a microfluidic chamber, showing TAPs’ transfer between a donor carrying the RP4 helper and the indicated TAP, and the E2 recipient strains. Real-time monitoring allows the visualization of the effect of TAP acquisition in the absence and presence of exposure to Ctx treatment. Arrows indicate Ctx S TAP donors producing both red and green fluorescence (red D), and Ctx R recipients producing no fluorescence (white R). TAP acquisition is reflected by the production of green fluorescence in transconjugant cells (green T). Time in minutes, with 0 min corresponding to the time of Ctx injection. Scale bar 1 μm. ( B ) Model for TAP activity against strains carrying bla CTX-M-15 . The donor strain transfers a TAP carrying cas9 or dcas9 genes through the conjugation machinery provided by the helper plasmid. The recipient cell having acquired the TAP produces the components of the CRISPR system. The Cas9 or the dCas9 protein is recruited to the targeted bla CTX-M-15 gene by the complementary gRNA. The Cas9 induces a DSB, while the dCas9 inhibits the expression of the bla CTX-M-15 gene. The subsequent effect strictly depends on the chromosomic or plasmidic location of the bla CTX-M-15 gene. TAP-Cas9 targeting the chromosome leads to cell death. TAP-Cas9 targeting a plasmid results in plasmid loss, which can lead to cell death if some TA systems are activated. TAP-dCas9 targeting the chromosome or a plasmid does not affect viability but results in resensitization of the recipient to the drug.
Wide Field Microscopy, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
wide field microscopy - by Bioz Stars, 2026-06
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wide field microscopy  (Nikon)
99
Nikon wide field microscopy
Visualization and model of TAP activity. ( A ) <t>Fluorescence</t> <t>microscopy</t> images of time-lapse experiments in a microfluidic chamber, showing TAPs’ transfer between a donor carrying the RP4 helper and the indicated TAP, and the E2 recipient strains. Real-time monitoring allows the visualization of the effect of TAP acquisition in the absence and presence of exposure to Ctx treatment. Arrows indicate Ctx S TAP donors producing both red and green fluorescence (red D), and Ctx R recipients producing no fluorescence (white R). TAP acquisition is reflected by the production of green fluorescence in transconjugant cells (green T). Time in minutes, with 0 min corresponding to the time of Ctx injection. Scale bar 1 μm. ( B ) Model for TAP activity against strains carrying bla CTX-M-15 . The donor strain transfers a TAP carrying cas9 or dcas9 genes through the conjugation machinery provided by the helper plasmid. The recipient cell having acquired the TAP produces the components of the CRISPR system. The Cas9 or the dCas9 protein is recruited to the targeted bla CTX-M-15 gene by the complementary gRNA. The Cas9 induces a DSB, while the dCas9 inhibits the expression of the bla CTX-M-15 gene. The subsequent effect strictly depends on the chromosomic or plasmidic location of the bla CTX-M-15 gene. TAP-Cas9 targeting the chromosome leads to cell death. TAP-Cas9 targeting a plasmid results in plasmid loss, which can lead to cell death if some TA systems are activated. TAP-dCas9 targeting the chromosome or a plasmid does not affect viability but results in resensitization of the recipient to the drug.
Wide Field Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wide field microscopy - by Bioz Stars, 2026-06
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axio imager a2 wide field fluorescence microscopy  (Carl Zeiss)
97
Carl Zeiss axio imager a2 wide field fluorescence microscopy
Visualization and model of TAP activity. ( A ) <t>Fluorescence</t> <t>microscopy</t> images of time-lapse experiments in a microfluidic chamber, showing TAPs’ transfer between a donor carrying the RP4 helper and the indicated TAP, and the E2 recipient strains. Real-time monitoring allows the visualization of the effect of TAP acquisition in the absence and presence of exposure to Ctx treatment. Arrows indicate Ctx S TAP donors producing both red and green fluorescence (red D), and Ctx R recipients producing no fluorescence (white R). TAP acquisition is reflected by the production of green fluorescence in transconjugant cells (green T). Time in minutes, with 0 min corresponding to the time of Ctx injection. Scale bar 1 μm. ( B ) Model for TAP activity against strains carrying bla CTX-M-15 . The donor strain transfers a TAP carrying cas9 or dcas9 genes through the conjugation machinery provided by the helper plasmid. The recipient cell having acquired the TAP produces the components of the CRISPR system. The Cas9 or the dCas9 protein is recruited to the targeted bla CTX-M-15 gene by the complementary gRNA. The Cas9 induces a DSB, while the dCas9 inhibits the expression of the bla CTX-M-15 gene. The subsequent effect strictly depends on the chromosomic or plasmidic location of the bla CTX-M-15 gene. TAP-Cas9 targeting the chromosome leads to cell death. TAP-Cas9 targeting a plasmid results in plasmid loss, which can lead to cell death if some TA systems are activated. TAP-dCas9 targeting the chromosome or a plasmid does not affect viability but results in resensitization of the recipient to the drug.
Axio Imager A2 Wide Field Fluorescence Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wide-field fluorescence microscopy thermo evos  (Thermo Fisher)
90
Thermo Fisher wide-field fluorescence microscopy thermo evos
Visualization and model of TAP activity. ( A ) <t>Fluorescence</t> <t>microscopy</t> images of time-lapse experiments in a microfluidic chamber, showing TAPs’ transfer between a donor carrying the RP4 helper and the indicated TAP, and the E2 recipient strains. Real-time monitoring allows the visualization of the effect of TAP acquisition in the absence and presence of exposure to Ctx treatment. Arrows indicate Ctx S TAP donors producing both red and green fluorescence (red D), and Ctx R recipients producing no fluorescence (white R). TAP acquisition is reflected by the production of green fluorescence in transconjugant cells (green T). Time in minutes, with 0 min corresponding to the time of Ctx injection. Scale bar 1 μm. ( B ) Model for TAP activity against strains carrying bla CTX-M-15 . The donor strain transfers a TAP carrying cas9 or dcas9 genes through the conjugation machinery provided by the helper plasmid. The recipient cell having acquired the TAP produces the components of the CRISPR system. The Cas9 or the dCas9 protein is recruited to the targeted bla CTX-M-15 gene by the complementary gRNA. The Cas9 induces a DSB, while the dCas9 inhibits the expression of the bla CTX-M-15 gene. The subsequent effect strictly depends on the chromosomic or plasmidic location of the bla CTX-M-15 gene. TAP-Cas9 targeting the chromosome leads to cell death. TAP-Cas9 targeting a plasmid results in plasmid loss, which can lead to cell death if some TA systems are activated. TAP-dCas9 targeting the chromosome or a plasmid does not affect viability but results in resensitization of the recipient to the drug.
Wide Field Fluorescence Microscopy Thermo Evos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Visualization and model of TAP activity. ( A ) Fluorescence microscopy images of time-lapse experiments in a microfluidic chamber, showing TAPs’ transfer between a donor carrying the RP4 helper and the indicated TAP, and the E2 recipient strains. Real-time monitoring allows the visualization of the effect of TAP acquisition in the absence and presence of exposure to Ctx treatment. Arrows indicate Ctx S TAP donors producing both red and green fluorescence (red D), and Ctx R recipients producing no fluorescence (white R). TAP acquisition is reflected by the production of green fluorescence in transconjugant cells (green T). Time in minutes, with 0 min corresponding to the time of Ctx injection. Scale bar 1 μm. ( B ) Model for TAP activity against strains carrying bla CTX-M-15 . The donor strain transfers a TAP carrying cas9 or dcas9 genes through the conjugation machinery provided by the helper plasmid. The recipient cell having acquired the TAP produces the components of the CRISPR system. The Cas9 or the dCas9 protein is recruited to the targeted bla CTX-M-15 gene by the complementary gRNA. The Cas9 induces a DSB, while the dCas9 inhibits the expression of the bla CTX-M-15 gene. The subsequent effect strictly depends on the chromosomic or plasmidic location of the bla CTX-M-15 gene. TAP-Cas9 targeting the chromosome leads to cell death. TAP-Cas9 targeting a plasmid results in plasmid loss, which can lead to cell death if some TA systems are activated. TAP-dCas9 targeting the chromosome or a plasmid does not affect viability but results in resensitization of the recipient to the drug.

Journal: Nucleic Acids Research

Article Title: Specific killing and resensitization of pathogenic Escherichia coli strains carrying bla CTX-M-15 β-lactamase using targeted-antibacterial-plasmids (TAPs)

doi: 10.1093/nar/gkaf1466

Figure Lengend Snippet: Visualization and model of TAP activity. ( A ) Fluorescence microscopy images of time-lapse experiments in a microfluidic chamber, showing TAPs’ transfer between a donor carrying the RP4 helper and the indicated TAP, and the E2 recipient strains. Real-time monitoring allows the visualization of the effect of TAP acquisition in the absence and presence of exposure to Ctx treatment. Arrows indicate Ctx S TAP donors producing both red and green fluorescence (red D), and Ctx R recipients producing no fluorescence (white R). TAP acquisition is reflected by the production of green fluorescence in transconjugant cells (green T). Time in minutes, with 0 min corresponding to the time of Ctx injection. Scale bar 1 μm. ( B ) Model for TAP activity against strains carrying bla CTX-M-15 . The donor strain transfers a TAP carrying cas9 or dcas9 genes through the conjugation machinery provided by the helper plasmid. The recipient cell having acquired the TAP produces the components of the CRISPR system. The Cas9 or the dCas9 protein is recruited to the targeted bla CTX-M-15 gene by the complementary gRNA. The Cas9 induces a DSB, while the dCas9 inhibits the expression of the bla CTX-M-15 gene. The subsequent effect strictly depends on the chromosomic or plasmidic location of the bla CTX-M-15 gene. TAP-Cas9 targeting the chromosome leads to cell death. TAP-Cas9 targeting a plasmid results in plasmid loss, which can lead to cell death if some TA systems are activated. TAP-dCas9 targeting the chromosome or a plasmid does not affect viability but results in resensitization of the recipient to the drug.

Article Snippet: Ctx was added and cells were imaged every 10 min for 3 h. Conventional wide-field fluorescence microscopy imaging was carried out on an Eclipse Ti2-E microscope (Nikon), equipped with x100/1.45 oil Plan Apo Lambda phase objective, ORCA-Fusion digital CMOS camera (Hamamatsu), and using NIS software for image acquisition.

Techniques: Activity Assay, Fluorescence, Microscopy, Injection, Conjugation Assay, Plasmid Preparation, CRISPR, Expressing